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recombinant active src kinase  (Sino Biological)


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    Structured Review

    Sino Biological recombinant active src kinase
    Recombinant Active Src Kinase, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+src/SRC%2C+Active/pm41068561-55-0-4
    Average 93 stars, based on 10 article reviews
    recombinant active src kinase - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells.
    Article Snippet: Recombinant biotinylated peptide fragments of ITGB1 (Genscript; Bio-ITGB1_27aa_tail: NAKWDTGENPIYKSAVTTVVNPKYEGK or Bio-ITGB1Y783P_27aa_tail: NAKWDTGENPI[pY]KSAVTTVVNPKYEGK) were then added to wells and allowed to bind to the streptavidin coating for 1 h at room temperature. .. The plates were then washed 5× with 0.1% TBS-T before being treated for 30 min at 37 °C with recombinant Src (0.1 μg ml−1; SignalChem, S19-10G-10), with/without ATP (20 μM), in kinase buffer (HEPES, pH 7.5 (60 mM)/MgCl2 (5 mM)/DTT (1.25 mM)/MnCl2 (5 mM)/Na3VO4 (3 μM)) as indicated. .. The phosphorylated tyrosine was then detected by first incubating with a pY primary antibody (PY20; 1:1,000, BD Biosciences, 610000) at room temperature and then with a horse radish peroxidase-conjugated anti-mouse secondary (1:2,000, Cell Signaling Technology, 7076) for 1 h at room temperature on a plate shaker (45 rpm).

    Article Title: Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells
    Article Snippet: Recombinant biotinylated peptide fragments of ITGB1 (Genscript; Bio-ITGB1_27aa_tail: NAKWDTGENPIYKSAVTTVVNPKYEGK or Bio-ITGB1Y783P_27aa_tail: NAKWDTGENPI[pY]KSAVTTVVNPKYEGK) were then added to wells and allowed to bind to the streptavidin coating for 1 h at room temperature. .. The plates were then washed 5× with 0.1% TBS-T before being treated for 30 min at 37 °C with recombinant Src (0.1 μg ml −1 ; SignalChem, S19-10G-10), with/without ATP (20 μM), in kinase buffer (HEPES, pH 7.5 (60 mM)/MgCl 2 (5 mM)/DTT (1.25 mM)/MnCl 2 (5 mM)/Na 3 VO 4 (3 μM)) as indicated. .. The phosphorylated tyrosine was then detected by first incubating with a pY primary antibody (PY20; 1:1,000, BD Biosciences, 610000) at room temperature and then with a horse radish peroxidase-conjugated anti-mouse secondary (1:2,000, Cell Signaling Technology, 7076) for 1 h at room temperature on a plate shaker (45 rpm).



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